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Becton Dickinson facstar plustm
Facstar Plustm, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/facstar+plustm/facstar+plustm/pm30106156-40-24-26
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facstar plustm - by Bioz Stars, 2026-10
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Purification:

Article Title: Arrested B Lymphopoiesis and Persistence of Activated B Cells in Adult Interleukin 7 −/ − Mice
Article Snippet: Samples were analyzed on FACScanTM or FACSCaliburTM flow cytometers (Becton Dickinson), and dead cells were excluded with propidium iodide. .. Bone marrow precursor cells (B220 + IgM − or CD19 + IgM − ) were purified by cell sorting, using a FACStar PLUSTM (Becton Dickinson), to a purity >98%. .. The cells were then plated under limiting dilution conditions in 96-well plates (48 wells/dilution) at 100, 33, and 11 cells/well (in the case of B220 + IgM − ) or 10, 3.3, and 1.1 cells/well (in the case of (CD19 + IgM − ) onto irradiated (2,000 Gy) S17 stromal cells in culture medium (Opti-MEM [GIBCO BRL]), with 10% FCS, Pen/Strep, and βM-E), supplemented with excess IL-7 and Stem cell factor (c-kit ligand), as described previously .

FACS:

Article Title: Arrested B Lymphopoiesis and Persistence of Activated B Cells in Adult Interleukin 7 −/ − Mice
Article Snippet: Samples were analyzed on FACScanTM or FACSCaliburTM flow cytometers (Becton Dickinson), and dead cells were excluded with propidium iodide. .. Bone marrow precursor cells (B220 + IgM − or CD19 + IgM − ) were purified by cell sorting, using a FACStar PLUSTM (Becton Dickinson), to a purity >98%. .. The cells were then plated under limiting dilution conditions in 96-well plates (48 wells/dilution) at 100, 33, and 11 cells/well (in the case of B220 + IgM − ) or 10, 3.3, and 1.1 cells/well (in the case of (CD19 + IgM − ) onto irradiated (2,000 Gy) S17 stromal cells in culture medium (Opti-MEM [GIBCO BRL]), with 10% FCS, Pen/Strep, and βM-E), supplemented with excess IL-7 and Stem cell factor (c-kit ligand), as described previously .

Article Title: Active Crohn's Disease Patients Show a Distinctive Expansion of Circulating Memory CD4<sup>+</sup>CD45RO<sup>+</sup>CD28<sup>null</sup>T Cells
Article Snippet: In a previous study we found an expansion of circulating memory (CD45RO+) CD4+ T cells in patients with Crohn’s disease (CD).. The aim of this work was to investigate the phenotypic and functional characteristics of this T-cell subset in CD.. We analyzed in peripheral blood CD4+CD45RO+ T cells from CD patients the expression of surface markers associated to immune activation, costimulation, and apoptosis.

Article Title: Receptor that causes cell death and recombinant production thereof
Article Snippet: .. CD11c+ CD8α+ DC were then isolated at high purity (>95%) by fluorescence activated cell sorting (FACS) using a FACStar PlusTM (Becton Dickinson, San Jose, Calif.), and used for biological evaluation of AIR/Fc. ..

Article Title: Distinctive pattern of cytokine production and adhesion molecule expression in peripheral blood memory CD4+ T cells from patients with active Crohn's disease.
Article Snippet: An expansion of both circulating and intestinal lamina propria CD4+CD45RO+ T cells has been described in patients with Crohn’s disease.. We studied both the cytokine profile and the expression of adhesion molecules on this T-cell subset.. Peripheral blood CD4+CD45RO+ T cells from patients with Crohn’s disease (n = 45) were assessed by flow cytometry and RT-PCR methods.

Isolation:

Article Title: Active Crohn's Disease Patients Show a Distinctive Expansion of Circulating Memory CD4<sup>+</sup>CD45RO<sup>+</sup>CD28<sup>null</sup>T Cells
Article Snippet: In a previous study we found an expansion of circulating memory (CD45RO+) CD4+ T cells in patients with Crohn’s disease (CD).. The aim of this work was to investigate the phenotypic and functional characteristics of this T-cell subset in CD.. We analyzed in peripheral blood CD4+CD45RO+ T cells from CD patients the expression of surface markers associated to immune activation, costimulation, and apoptosis.

Article Title: Receptor that causes cell death and recombinant production thereof
Article Snippet: .. CD11c+ CD8α+ DC were then isolated at high purity (>95%) by fluorescence activated cell sorting (FACS) using a FACStar PlusTM (Becton Dickinson, San Jose, Calif.), and used for biological evaluation of AIR/Fc. ..

Staining:

Article Title: Active Crohn's Disease Patients Show a Distinctive Expansion of Circulating Memory CD4<sup>+</sup>CD45RO<sup>+</sup>CD28<sup>null</sup>T Cells
Article Snippet: In a previous study we found an expansion of circulating memory (CD45RO+) CD4+ T cells in patients with Crohn’s disease (CD).. The aim of this work was to investigate the phenotypic and functional characteristics of this T-cell subset in CD.. We analyzed in peripheral blood CD4+CD45RO+ T cells from CD patients the expression of surface markers associated to immune activation, costimulation, and apoptosis.

Article Title: Immune thrombocytopenia induces autophagy and suppresses apoptosis in megakaryocytes.
Article Snippet: .. Following this, cells were stained with Annexin V/PI (Beyotime Institute of Biotechnology, Haimen, China), according to the manufacturer's protocol and then analyzed with a FACStar PLUSTM (BD Biosciences, Franklin Lakes, NJ, USA). .. Evalua t ion of ce l l au tophagy by Lyso ‐Tracker Red/ansylcadaverine (MDC) assay and flow cytometry.

Article Title: Distinctive pattern of cytokine production and adhesion molecule expression in peripheral blood memory CD4+ T cells from patients with active Crohn's disease.
Article Snippet: An expansion of both circulating and intestinal lamina propria CD4+CD45RO+ T cells has been described in patients with Crohn’s disease.. We studied both the cytokine profile and the expression of adhesion molecules on this T-cell subset.. Peripheral blood CD4+CD45RO+ T cells from patients with Crohn’s disease (n = 45) were assessed by flow cytometry and RT-PCR methods.

Flow Cytometry:

Article Title: Anti-ErbB2 antibodies
Article Snippet: .. Cells were simultaneously examined for viability and cell cycle status by flow cytometry on a FACSTAR PLUSTM (Becton Dickinson Immunocytometry Systems USA, San Jose, Calif.). .. Breast tumor cells were harvested by washing the monolayer with phosphate buffered saline (PBS), incubating cells in 0.05% trypsin and 0.53 mM EDTA (Gibco) and resuspending them in culture medium.

Fluorescence:

Article Title: Receptor that causes cell death and recombinant production thereof
Article Snippet: .. CD11c+ CD8α+ DC were then isolated at high purity (>95%) by fluorescence activated cell sorting (FACS) using a FACStar PlusTM (Becton Dickinson, San Jose, Calif.), and used for biological evaluation of AIR/Fc. ..

Expressing:

Article Title: Ligand-independent Signaling Functions for the B Lymphocyte Antigen Receptor and Their Role in Positive Selection during B Lymphopoiesis
Article Snippet: .. Cells were sorted based upon GFP and CD45 expression directly into 96-well plates using a Becton Dickinson FACStar PLUSTM . ..



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Expression of RE2 epitope and cytotoxic sensitivity to mAb RE2 of the human HLA-A, B-negative EBV-transformed B cell line C1R, transfected with xeno-chimeric MHC class I genes composed of either murine H-2K b or human HLA-B7 at α1, α2, and α3 domains. Expression of RE2 molecules was analyzed by incubating on ice target cells with mAb RE2, followed by FITC-conjugated mouse anti–rat immunoglobulins, and cells were subjected to the analysis using <t>FACStar</t> PLUS™ . Cytotoxic sensitivity of transfectants to mAb RE2 was analyzed, as described in Materials and Methods (right column).
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Expression of RE2 epitope and cytotoxic sensitivity to mAb RE2 of the human HLA-A, B-negative EBV-transformed B cell line C1R, transfected with xeno-chimeric MHC class I genes composed of either murine H-2K b or human HLA-B7 at α1, α2, and α3 domains. Expression of RE2 molecules was analyzed by incubating on ice target cells with mAb RE2, followed by FITC-conjugated mouse anti–rat immunoglobulins, and cells were subjected to the analysis using FACStar PLUS™ . Cytotoxic sensitivity of transfectants to mAb RE2 was analyzed, as described in Materials and Methods (right column).

Journal: The Journal of Experimental Medicine

Article Title: A Monoclonal Antibody to the α2 Domain of Murine Major Histocompatibility Complex Class I that Specifically Kills Activated Lymphocytes and Blocks Liver Damage in the Concanavalin A Hepatitis Model

doi: 10.1084/jem.20021301

Figure Lengend Snippet: Expression of RE2 epitope and cytotoxic sensitivity to mAb RE2 of the human HLA-A, B-negative EBV-transformed B cell line C1R, transfected with xeno-chimeric MHC class I genes composed of either murine H-2K b or human HLA-B7 at α1, α2, and α3 domains. Expression of RE2 molecules was analyzed by incubating on ice target cells with mAb RE2, followed by FITC-conjugated mouse anti–rat immunoglobulins, and cells were subjected to the analysis using FACStar PLUS™ . Cytotoxic sensitivity of transfectants to mAb RE2 was analyzed, as described in Materials and Methods (right column).

Article Snippet: Antibiotic-resistant clones were isolated and expanded, and expression of the hybrid MHC class I molecules was confirmed, using flow cytometric analysis with FACStar PLUSTM (Becton Dickinson).

Techniques: Expressing, Transformation Assay, Transfection

Therapeutic effects of mAb RE2 on Con A–induced hepatitis in mice. (A) Serum levels of GOT and GPT in C57BL/6 mice ( n = 6) 24 h after an intravenous injection of Con A, with or without administration of mAb RE2. Closed and open bars represent serum levels of GOT and GPT, respectively. (B) Histopathology of massive liver cell necrosis seen in a mouse 24 h after an intravenous injection of Con A. (C) Absence of Con A-induced liver cell necrosis in a mouse treated with mAb RE2. (D) Reduction of CD69-positive activated CD4 + and CD8 + T cells and NK1.1 + CD3 + NKT cells in the liver of mice given Con A and mAb RE2 (bottom), compared with findings in mice given Con A alone (top). NKT cells were examined by triple staining of cells with mAbs to CD69, NK1.1, and CD3, and gated CD3 + cells were analyzed for CD69 and NK1.1, using FACStar PLUS™ .

Journal: The Journal of Experimental Medicine

Article Title: A Monoclonal Antibody to the α2 Domain of Murine Major Histocompatibility Complex Class I that Specifically Kills Activated Lymphocytes and Blocks Liver Damage in the Concanavalin A Hepatitis Model

doi: 10.1084/jem.20021301

Figure Lengend Snippet: Therapeutic effects of mAb RE2 on Con A–induced hepatitis in mice. (A) Serum levels of GOT and GPT in C57BL/6 mice ( n = 6) 24 h after an intravenous injection of Con A, with or without administration of mAb RE2. Closed and open bars represent serum levels of GOT and GPT, respectively. (B) Histopathology of massive liver cell necrosis seen in a mouse 24 h after an intravenous injection of Con A. (C) Absence of Con A-induced liver cell necrosis in a mouse treated with mAb RE2. (D) Reduction of CD69-positive activated CD4 + and CD8 + T cells and NK1.1 + CD3 + NKT cells in the liver of mice given Con A and mAb RE2 (bottom), compared with findings in mice given Con A alone (top). NKT cells were examined by triple staining of cells with mAbs to CD69, NK1.1, and CD3, and gated CD3 + cells were analyzed for CD69 and NK1.1, using FACStar PLUS™ .

Article Snippet: Antibiotic-resistant clones were isolated and expanded, and expression of the hybrid MHC class I molecules was confirmed, using flow cytometric analysis with FACStar PLUSTM (Becton Dickinson).

Techniques: Injection, Histopathology, Staining